TimeMaster™ Lifetime Microscopy

Fluorescence detection techniques are well established in cell biology. Fluorescence microscopy is widely used to visualize microscopic structures and functional properties of cellular organelles. One of the most important features of fluorescence techniques is that commercially available fluorescent probes can provide information about various properties of their local environment in the cell. They can be used to monitor pH, viscosity, concentration of metal ions, polarity, molecular oxygen, conformational changes of the host environment etc. Measurable parameters that are affected by these factors are the fluorescence spectrum, lifetime, quantum yield and anisotropy of the fluorophores. While the steady-state (intensity) measurement can reflect some of these changes, the time-resolved (lifetime) measurement will give a more detailed insight into the mechanism of the process under study. Lifetime-based measurements are especially desirable, because unlike the intensity, the fluorescence lifetime is independent of the local fluorophore concentration. The Stray light is less of a problem in lifetime measurements and the technique can also be used to differentiate between fluorescent probes with overlapping spectra but different lifetimes. The time-resolved technique is also a powerful tool to study chemical systems such as colloids, liquid crystals, polymers, phase transition temperatures etc.

Recommended applications include:

  • Fluorescence Lifetime Microscopy of Labeled Cells
  • Measurement of Intracellular Ions
  • Lifetime-based Oxygen Sensing
  • Lymphocytes Screening for Cancer
  • Multiphase Systems
  • Surface Photochemistry
  • Materials Research

Manufactured by HORIBA Scientific

Detection limit Sensitivity allows for the measurement of lifetimes of 7 pM fluorescein
Emission Range 185 to 680 nm (optional to 900 nm)
Detection Time domain, patented stroboscopic technique
Minimum measurable lifetime < 100 ps
Data acquisition rate < 30 seconds typ.
Wavelength range 360 to 990 nm (depending on dye used)
235 to 345 nm (with optional frequency doubler)
Pulse width 800 picoseconds
Peak power 220 microjoules per pulse at 500 nm, 5 Hz
Repetition range 2 to 20 Hz
Note: Specifications are based on standard system.

Hardware

1. Light Source

2. User Povided Microscope

3. Photometer

4. Detector

 
Detection limit Sensitivity allows for the measurement of lifetimes of 7 pM fluorescein
Emission Range 185 to 680 nm (optional to 900 nm)
Detection Time domain, patented stroboscopic technique
Minimum measurable lifetime < 100 ps
Data acquisition rate < 30 seconds typ.
Wavelength range 360 to 990 nm (depending on dye used)
235 to 345 nm (with optional frequency doubler)
Pulse width 800 picoseconds
Peak power 220 microjoules per pulse at 500 nm, 5 Hz
Repetition range 2 to 20 Hz
Note: Specifications are based on standard system.

 

No system would be complete without the ability to add options and accessories. PTI is a one-stop shop for upgrades, add-ons and accessories to complement your system.

Dual Channel Photometer

Has all the properties of a single channel photometer, plus it allows an addition of a gated phosphorescence detector for time-resolved lanthanide luminescence and LRET

Dual Channel Photometer

TCSPC Lifetime Flyer

Tags: Time-Resolved Spectrofluorometer, fluorescence lifetime, Biology, Microscope

PTI - Horiba

PT. HORIBA Indonesia

Ruko Jalur Sutera Jl. Jalur Sutera Blok 20 A No. 16-17 Tangerang, Banten 15144, Indonesia

Tel: +62 (21) 30448525
Fax: +62 (21) 30448521
Email: info-sci.id@horiba.com