Equipped with a computer-controlled scanning emission monochromator for instant wavelength selection and direct time-resolved spectra acquisition, the TM-2000 provides impressive wavelength coverage from 260 nm to 670 nm. The QuadraCentric™ sample compartment comes standard with a 10 x 10 mm cuvette holder. The compartment is roomy and will accept various options such as polarizers, solid or powdered sample holders, and other accessories. The TM-2000 is suitable for virtually all fluorescence lifetime applications.

Manufactured by HORIBA Scientific

TimeMaster™ 50 comes with FelixGX software for instrumental control and includes new PowerFit-10 analytical software for fluorescence and phosphorescence lifetime analysis.

Through the new, powerful ASOC-10 USB interface FelixGX provides a full set of data acquisition protocols and controls the hardware for all system configurations and operating modes.

FelixGX Controls

• Trigger and rep rate of pulsed laser
• Timing of digital detector delay gate
• Monochromator (optional)
• Motorized slits (optional)

FelixGX Acquisition Modes

FelixGX also provides several acquisition modes for fluorescence decay measurements:

• Fluorescence Decay with user controlled laser reprate, number of pulses averaged per point and user selectable linear, arithmetic or logarithmic timescale
• Time-Resolved Emission scan (if optional emission monochromator is present) with user control of laser reprate, time delay of detector gate, number of pulses averaged per wavelength and scan step size  
• Timebased scan with user control of laser reprate, time delay of detector gate, total time window and number of pulses averaged per point

PowerFit-10 Analytical Software

PowerFit-10 software (incorporated into FelixGX) provides powerful analytical package for decay data analysis. All modules include reconvolution algorithms, selection of data weighing (Poisson or analog), shift and offset parameters, statistical goodness-of-fit parameters (Chi-square, Durbin-Watson, Runs test, residuals and autocorrelation), as well as standard deviations of fit parameters.

PowerFit-10 Modules

• Multi-exponential (1 to 4 lifetimes) decay
• Multi-file multi-exponential (1 to 4 lifetimes)
• Global (1 to 4 lifetimes) analysis
• Anisotropy decay (free rotor, restricted rotor and other models)
• Stretched exponential
• Micelle quenching kinetics (Infelta-Groetzel-Tachiya model)
• Exponential Series Method (ESM) unconstrained lifetime distribution analysis (up to 200 exp terms)
• Maximum Entropy Method (MEM) lifetime distribution analysis (up to 200 exp terms)
• Time-Resolved Spectra (TRES)
• Decay Associated Spectra (DAS)

Fluorescence decay from a small area (5 x 5 micron) of fluorescein-labeled bead bracketed by photometer