TimeMaster™ LED

Equipped with a computer-controlled scanning emission monochromator for instant wavelength selection and direct time-resolved spectra acquisition, the TM-2000 provides impressive wavelength coverage from 260 nm to 670 nm. The QuadraCentric™ sample compartment comes standard with a 10 x 10 mm cuvette holder. The compartment is roomy and will accept various options such as polarizers, solid or powdered sample holders, and other accessories. The TM-2000 is suitable for virtually all fluorescence lifetime applications.

Manufactured by HORIBA Scientific

Lifetime Range 100 ps to 3 μs
Excitation Range 260 to 670 nm
Pulse Width 1.5 ns (typical)
Emission Range 185 to 680 nm (optional to 900 nm)
Detection Time domain, patented stroboscopic technique
Sensitivity < 1 nM fluorescein, approx. 10 nM NATA
Acquisition Time 20 s (sample dependent)
Time Scale Linear, arithmetic progression and logarithmic
Acquisition Mode Sequential or random
Note: Specifications are based on standard system.
 

TimeMaster™ 50 comes with FelixGX software for instrumental control and includes new PowerFit-10 analytical software for fluorescence and phosphorescence lifetime analysis.

Through the new, powerful ASOC-10 USB interface FelixGX provides a full set of data acquisition protocols and controls the hardware for all system configurations and operating modes.

FelixGX Controls

  • Trigger and rep rate of pulsed laser
  • Timing of digital detector delay gate
  • Monochromator (optional)
  • Motorized slits (optional)

FelixGX Acquisition Modes

FelixGX also provides several acquisition modes for fluorescence decay measurements:

  • Fluorescence Decay with user controlled laser reprate, number of pulses averaged per point and user selectable linear, arithmetic or logarithmic timescale
  • Time-Resolved Emission scan (if optional emission monochromator is present) with user control of laser reprate, time delay of detector gate, number of pulses averaged per wavelength and scan step size  
  • Timebased scan with user control of laser reprate, time delay of detector gate, total time window and number of pulses averaged per point

PowerFit-10 Analytical Software

PowerFit-10 software (incorporated into FelixGX) provides powerful analytical package for decay data analysis. All modules include reconvolution algorithms, selection of data weighing (Poisson or analog), shift and offset parameters, statistical goodness-of-fit parameters (Chi-square, Durbin-Watson, Runs test, residuals and autocorrelation), as well as standard deviations of fit parameters.

PowerFit-10 Modules

  • Multi-exponential (1 to 4 lifetimes) decay
  • Multi-file multi-exponential (1 to 4 lifetimes)
  • Global (1 to 4 lifetimes) analysis
  • Anisotropy decay (free rotor, restricted rotor and other models)
  • Stretched exponential
  • Micelle quenching kinetics (Infelta-Groetzel-Tachiya model)
  • Exponential Series Method (ESM) unconstrained lifetime distribution analysis (up to 200 exp terms)
  • Maximum Entropy Method (MEM) lifetime distribution analysis (up to 200 exp terms)
  • Time-Resolved Spectra (TRES)
  • Decay Associated Spectra (DAS)

 

Fluorescence decay from a small area (5 x 5 micron) of fluorescein-labeled bead bracketed by photometer

T-2 Steady State Upgrade

Add steady state measurement to lifetime systems. More..

T-1a Phosphorescence Upgrade

Add phosphoresence measurement to lifetime systems. More..

TCSPC Lifetime Flyer
 

Fluorescence Lifetime Microscopy with TM-5 Laser-Based Fluorometer

Chinese hamster ovary cell

A single Chinese hamster ovary cell labeled with monobromotrimethylammonio-bimane (QBBr). A: QBBr-labeled cell in standard Ringer’s solution; B: same cell, but with 160 mM NaI substituted for NaCl in Ringer’s solution (note quenching by I- ions); C: same cell back to standard Ringer’s solution (note the recovery of fluorescence lifetime). The decay curves measured with a TM-5 microscope based lifetime system. Data courtesy of Prof. Brad S. Rothberg, Dept. Physiology, Univ. of Texas, San Antonio.

Tags: Fluorescence, Lifetime, LED, Time-Resolved Spectrofluorometer, Strobe

PTI - Horiba

PT. HORIBA Indonesia

Ruko Jalur Sutera Jl. Jalur Sutera Blok 20 A No. 16-17 Tangerang, Banten 15144, Indonesia

Tel: +62 (21) 30448525
Fax: +62 (21) 30448521
Email: info-sci.id@horiba.com