PicoMaster TCSPC Lifetime Fluorometer

Short lifetimes

Ability to measure short lifetimes is determined by the excitation pulse width and the temporal response of the detector. Fast PMT detectors offered with PicoMaster  make it possible to measure shorter lifetimes than with conventional side-on PMTs. The pulse width of our laser diodes is typically 50-60 ps (laser diode dependent), while PMD-1, PMD-2 and PMD-3C detectors have a response time of approx. 180 ps and are well designed to provide a uniform response over the entire area of the photocathode. Even shorter instrument response function is possible if a picosecond mode-locked laser and an MCP detector are used. In the latter case, lifetimes down to less than 10 ps can be deconvoluted with PTI's analytical software.

High dynamic range

One of the main strengths of PTI's PicoMaster is the high dynamic range for the decay curve acquisition. Typically decays can be acquired up to 4-5 decades of the photon count range. While the PicoMaster  lacks the ability of the TM-3000 and TM-2000 to acquire decays over a logarithmic time scale, the high dynamic range and the narrow instrument response function enables the user to determine the lifetime of very small fractions of fluorophore in a mixture. It also makes numerical fitting more precise for multiple lifetime analysis.

Versatility

The PicoMaster's open architecture design allows for many options, which can be added at any time as you needed or as your budget can afford. Partial lists of what you can add are: choices in light sources, single or dual emission, fluorescence or phosphorescence lifetime capability, coupling to a microscope.  At your request we are constantly working to bring you more options, continually expanding the versatility of the TimeMaster™ series.

Manufactured by HORIBA Scientific

Detection Technique Time-Correlated Single Photon Counting (TCSPC)
Light Source
ns LEDs and LDs:

ps LDs:
266, 280, 297, 310, 340, 368, 375, 403, 407, 432, 444, 456, 486, 510, 633, 649, 667 nm
375, 405, 445, 473, 488, 635, 650, 660, 670 nm (up to 1550 nm available)

(Can accommodate other LEDs, LDs, fs-ps mode-locked, supercontinuum fiber and other high rep rate lasers)

Rep Rate LEDs: up to 180 KHz (LED-dependent) 
LDs: 1, 20, 50 MHz (up to 80 MHz available)
Pulse Width LEDs: < 1.5 ns ps 
LDs: down to 50 ps
PMT Response 180 ps (UV-VIS), 300 ps (NIR)
Temporal Resolution 813 fs
Shortest Lifetime < 10 ps (with fs/ps mode-locked laser and MCP)
Emission Spectral Range 180–1700 nm (detector dependent)

 

Hardware

PicoMaster 1 TCSPC Lifetime Fluorometer Block Diagram

1. Light Source

2. Sample compartment

3. Adjustable slits

4. Monochromator

5. Grating

6. Detector

7. TCSPC Board

 

 

System Specifications

Detection Technique Time-Correlated Single Photon Counting (TCSPC)
Light Source
ns LEDs and LDs:

ps LDs:
266, 280, 297, 310, 340, 368, 375, 403, 407, 432, 444, 456, 486, 510, 633, 649, 667 nm
375, 405, 445, 473, 488, 635, 650, 660, 670 nm (up to 1550 nm available)

(Can accommodate other LEDs, LDs, fs-ps mode-locked, supercontinuum fiber and other high rep rate lasers)

Rep Rate LEDs: up to 180 KHz (LED-dependent) 
LDs: 1, 20, 50 MHz (up to 80 MHz available)
Pulse Width LEDs: < 1.5 ns ps 
LDs: down to 50 ps
PMT Response 180 ps (UV-VIS), 300 ps (NIR)
Temporal Resolution 813 fs
Shortest Lifetime < 10 ps (with fs/ps mode-locked laser and MCP)
Emission Spectral Range 180–1700 nm (detector dependent)

 

Phosphorescence Enhancement T-1B

 

 

Add phosphorescence lifetime and steady state capability to a lifetime system. Includes: pulsed xenon source, excitation monochromator, and multi-mode PMT detector. One can select detectors by means of a flipping mirror.

  T-1B Upgrade to PTI's PicoMaster TCSPC Lifetime Fluorometer
 
 

Steady State Enhancement T-2B

 

 

Add steady state measurement capability to a lifetime system. Includes: high intensity continuous xenon source, excitation monochromator and new multi-mode PMT detector. One can then choose between detectors by means of a flipping mirror.

  T-2B Upgrade to PTI's PicoMaster TCSPC Lifetime Fluorometer
 
 

Phosphorescence Light Source Enhancement Q-4G

 

 

Add a pulsed xenon light source to enable phosphorescence lifetime measurement capability. Requires T-2B

  Q-4G Upgrade to PTI's PicoMaster TCSPC Lifetime Fluorometer
 
 

Steady State Light Source Enhancement Q-5H

 

 

Add a high intensity "Green" (high efficiency low consumption) continuous xenon source for excitation. Requires T-1B

  Q-5H Upgrade to PTI's PicoMaster 1 TCSPC Lifetime Fluorometerr

PicoMaster TCSPC system comes with FelixGX software for instrumental control and the new PowerFit-10 analytical software is available for fluorescence lifetime analysis.

Through the new, powerful ASOC-10 USB interface FelixGX provides a full set of data acquisition protocols and controls the hardware for all system configurations and operating modes.

FelixGX controls:

  • Monochromators
  • Motorized slits
  • Motorized polarizers
  • Motorized sample holders
  • Temperature control Peltier devices
  • Detectors
  • External devices such as stopped flow

FelixGX also fully controls all functions of the TCSPC board:

  • Time-to-Amplitude Converter (TAC) range, gain, offset, limit low and limit high
  • Sync and Stop channel CFD thresholds and crossings
  • Time delay of Sync pulse - number of channels
  • Frequency divider
  • Acquisition stop method (stop button, peak channel count or time)

PowerFit-10

PowerFit-10 software (incorporated into FelixGX) provides powerful analytical package for decay data analysis. All modules include reconvolution algorithms, selection of data weighing (Poisson or analog), shift and offset parameters, statistical goodness-of-fit parameters (Chi-square, Durbin-Watson, Runs test, residuals and autocorrelation), as well as standard deviations of fit parameters.

PowerFit-10 contains the following modules:

  • multi-exponential (1 to 4 lifetimes) decay
  • multi-file multi-exponential (1 to 4 lifetimes)
  • global (1 to 4 lifetimes) analysis
  • anisotropy decay (free rotor, restricted rotor and other models)
  • stretched exponential
  • micelle quenching kinetics (Infelta-Groetzel-Tachiya model)
  • Exponential Series Method (ESM) unconstrained lifetime distribution analysis (up to 200 exp terms)
  • Maximum Entropy Method (MEM) lifetime distribution analysis (up to 200 exp terms)
  • Time-Resolved Spectra (TRES)
  • Decay Associated Spectra (DAS)

 

FelixGX TCSPC Software

 

FelixGX TCSPC

Stopped Flow Accessory

The stopped flow accessory is used to rapidly mix small volumes of two (or more) different chemicals in a cuvette, quickly stop the flow of chemicals to the cuvette and monitor the resulting chemical reaction via optical means.

Download Flyer (174 KB PDF)

PTI LaserStrobe Stopped Flow Accessory
 

Four-position Sample Holder

The muli-position Peltier, featuring a four-position turret and magnetic stirring can accommodate standard cuvettes or microcuvettes. The automated sample holder has a controllable temperature range of -20°C to 105°C.

PTI LaserStrobe Four Position Solid Sample Holder
 

Powdered Sample Holder

The powdered sample holder head easily adapts to the solid sample base with one thumbscrew adjustment. The sample head can be disassembled for easy sample loading and cleaning.

Download Flyer (146 KB PDF)

PTI LaserStrobe Powdered Sample Holder
 

Front Face Solid Sample Holder

The front face solid sample holder, capable of both linear and rotational travel, was designed for the measurements of solid compounds, microscope slides, or films. The solid sample holder head mounts onto a base that can be removed easily to substitute a powder sample holder head.

Download Flyer (146 KB PDF)

PTI LaserStrobe Solid Sample Holder
 

Titrators

Titrations are performed to measure a number of biochemical and physical parameters, including binding constants, stoichiometry and kinetics. PTI offers fully automated titration solutions that are integrated into the software. Parameters such as mixing, volume, speed, and calibration are dictated in the software and can be adapted to your needs.

 

Integrating Sphere

Redesigned for enhanced measurement of quantum yields of solids, films, and powders. We use a 6 inch diameter sphere and attach it directly to the sample chamber on the port opposite the excitation channel. This design minimizes the effect of the excitation, emission, and sampling ports on the accuracy of the measurement.

Download Flyer (432 KB PDF)

PTI LaserStrobe Intergrating Sphere
 

Single Cuvette Peltier

The Peltier provides unmatched temperature stability and accuracy over the controllable temperature range of -20°C to 105°C.

PTI--QuantaMaster Luminescence Single Cuvette Pelitier
 

PTI QuantaMaster Luminsecence Muscle Strip AccessoryMuscle Strip

The muscle strip is inserted into a standard 1 cm cuvette, and can be used with any cuvette-based fluorescence system having a standard single cuvette holder complete with tension transducer and transducer mounting bracket with micrometer position adjustment.

 

Polarizers

PTI offers a wide variety of polarizers ranging from manual sheet polarizers (view graph) to automated large aperture Glan Thompson polarizers (view graph).

PTI LaserStrobe Polarizers
 

Cold Finger Dewar

The cold finger dewar is designed to be used with liquid nitrogen as coolant (77 K). It can also be used with organic solvent slushes at discrete temperatures above 77 K.

Download Flyer (126 KB PDF)

PTI LaserStrobe Cold Finger Dewar
 

Remote Sensing Accessory

The remote sensing accessory allows in vitro or in vivo measurement by means of a quartz bifurcated fiber bundle or Liquid Light Guide.

 

Selections of Long-pass Filters

Long-pass filter sets provide spectral discrimination for the emission channel and prevent the excitation light from being detected by the PMT. Filters should be selected so they cut off  unwanted wavelengths, such the second order.  The filters should be used with a filter holder (2 filter holders included with instrument).

The longpass filter set includes 1 inch round filters with cut-off wavelengths of 345, 400, 445, and 515 nm

 

PTI LaserStrobe Filters
 

Bandpass Filters

10 nm bandpass interference filters are available individually or as a set. The peak transmittance wavelengths are 280, 289, 297, 308, 334 nm and from 340 nm to 650 nm at 10 nm increments. The filters should be used with a filter holder (2 filter holders included with instrument). The bandpass filters are especially recommended for:

  • FRET experiments when looking at donor fluorescence
  • Samples which exhibit fluorescent tautomeric forms
  • Excimer and exciplex systems, when looking at the monomer emission band
  • Molecules, which undergo excited state proton transfer (ESPT) or intramolecular charge transfer (ICT, TICT) and exhibit dual fluorescence bands
  • Experiments, which require elimination of unwanted emission at longer wavelengths
 

Neutral Density Filters

Neutral density filter set. The neutral density filter set consists of six 1inch round filters with approximate optical densities of 0.1, 0.3, 0.5, 1.0, 2.0 and 3.0 suitable for a spectral range of 300–2000 nm. The filters should be used with a filter holder (2 filter holders included with instrument). The neutral density filters are used to decrease the signal intensity without changing the width of the emission slit.

PTI LaserStrobe Filter and Holder
 

Semimicro Cuvette

The semi-microcuvette cuvette and insert lets the end user save money and time by using smaller volumes of samples while maintaining the correct optical height.

Fluorescence Lifetime Brochure
 
TCSPC Lifetime Flyer
 

Whether you’re working in biology, medicine, physical or material science, measuring proteins, DNA, FRET and others, the PicoMaster TCSPC lifetime spectrofluorometer is the instrument for you! And with PTI's accessories and system enhancements the PicoMaster the perfect system for any fluorescence lifetime application.

Below are some examples:

  • Intrinsic protein fluorescence
  • FRET
  • Quantum dots
  • DNA binding
  • Fluorescence lifetimes
  • Protein-protein interactions
  • Protein-drug interactions
  • Anisotropy decays
  • Molecular photophysics
  • Photosynthesis
  • Semiconductors, glasses and ceramics

 

Leaf Deacy with PicoMaster 1
The high dynamic range of PicoMaster greatly facilitates the determination of minor lifetime components in the complex decay of native chlorophyll in photosynthetic system of green leaves

 

Protein Anistropy of BSA
PicoMaster is an excellent choice for unraveling structural and dynamic properties of proteins by measuring rotational correlation times from anisotropy decays

Tags: Life time, fluorescence lifetime, luminescence, TCSPC

PTI - Horiba

PT. HORIBA Indonesia

Ruko Jalur Sutera Jl. Jalur Sutera Blok 20 A No. 16-17 Tangerang, Banten 15144, Indonesia

Tel: +62 (21) 30448525
Fax: +62 (21) 30448521
Email: info-sci.id@horiba.com