- HOME PAGE
- Message from the President
- Corporate Philosophy
- Corporate Culture
- Technical Expertise
- Worldwide Locations
- UN Global Compact
- Basis for Management
- Integrated Management System
- Environmental Policies
- Actions for RoHS Directive, REACH Regulation and GHS Regulations
- Material Procurement
- Promotion of Diversity
- Social Activities
- MOLECULAR SPECTROSCOPY
- ELEMENTAL ANALYSIS
- SPECTROSCOPY SOLUTION
- SURFACE CHARACTERIZATION
- PARTICLE CHARACTERIZATION
- ENVIRONMENTAL ANALYSIS
- FORENSICS EQUIPMENT
- Technical document
- News and events
PicoMaster TCSPC Lifetime Fluorometer
Ability to measure short lifetimes is determined by the excitation pulse width and the temporal response of the detector. Fast PMT detectors offered with PicoMaster make it possible to measure shorter lifetimes than with conventional side-on PMTs. The pulse width of our laser diodes is typically 50-60 ps (laser diode dependent), while PMD-1, PMD-2 and PMD-3C detectors have a response time of approx. 180 ps and are well designed to provide a uniform response over the entire area of the photocathode. Even shorter instrument response function is possible if a picosecond mode-locked laser and an MCP detector are used. In the latter case, lifetimes down to less than 10 ps can be deconvoluted with PTI's analytical software.
High dynamic range
One of the main strengths of PTI's PicoMaster is the high dynamic range for the decay curve acquisition. Typically decays can be acquired up to 4-5 decades of the photon count range. While the PicoMaster lacks the ability of the TM-3000 and TM-2000 to acquire decays over a logarithmic time scale, the high dynamic range and the narrow instrument response function enables the user to determine the lifetime of very small fractions of fluorophore in a mixture. It also makes numerical fitting more precise for multiple lifetime analysis.
The PicoMaster's open architecture design allows for many options, which can be added at any time as you needed or as your budget can afford. Partial lists of what you can add are: choices in light sources, single or dual emission, fluorescence or phosphorescence lifetime capability, coupling to a microscope. At your request we are constantly working to bring you more options, continually expanding the versatility of the TimeMaster™ series.
Phosphorescence Enhancement T-1B
Add phosphorescence lifetime and steady state capability to a lifetime system. Includes: pulsed xenon source, excitation monochromator, and multi-mode PMT detector. One can select detectors by means of a flipping mirror.
Add steady state measurement capability to a lifetime system. Includes: high intensity continuous xenon source, excitation monochromator and new multi-mode PMT detector. One can then choose between detectors by means of a flipping mirror.
Add a pulsed xenon light source to enable phosphorescence lifetime measurement capability. Requires T-2B
Add a high intensity "Green" (high efficiency low consumption) continuous xenon source for excitation. Requires T-1B
PicoMaster TCSPC system comes with FelixGX software for instrumental control and the new PowerFit-10 analytical software is available for fluorescence lifetime analysis.
Through the new, powerful ASOC-10 USB interface FelixGX provides a full set of data acquisition protocols and controls the hardware for all system configurations and operating modes.
- Motorized slits
- Motorized polarizers
- Motorized sample holders
- Temperature control Peltier devices
- External devices such as stopped flow
FelixGX also fully controls all functions of the TCSPC board:
- Time-to-Amplitude Converter (TAC) range, gain, offset, limit low and limit high
- Sync and Stop channel CFD thresholds and crossings
- Time delay of Sync pulse - number of channels
- Frequency divider
- Acquisition stop method (stop button, peak channel count or time)
PowerFit-10 software (incorporated into FelixGX) provides powerful analytical package for decay data analysis. All modules include reconvolution algorithms, selection of data weighing (Poisson or analog), shift and offset parameters, statistical goodness-of-fit parameters (Chi-square, Durbin-Watson, Runs test, residuals and autocorrelation), as well as standard deviations of fit parameters.
PowerFit-10 contains the following modules:
- multi-exponential (1 to 4 lifetimes) decay
- multi-file multi-exponential (1 to 4 lifetimes)
- global (1 to 4 lifetimes) analysis
- anisotropy decay (free rotor, restricted rotor and other models)
- stretched exponential
- micelle quenching kinetics (Infelta-Groetzel-Tachiya model)
- Exponential Series Method (ESM) unconstrained lifetime distribution analysis (up to 200 exp terms)
- Maximum Entropy Method (MEM) lifetime distribution analysis (up to 200 exp terms)
- Time-Resolved Spectra (TRES)
- Decay Associated Spectra (DAS)
Stopped Flow Accessory
The stopped flow accessory is used to rapidly mix small volumes of two (or more) different chemicals in a cuvette, quickly stop the flow of chemicals to the cuvette and monitor the resulting chemical reaction via optical means.
Download Flyer (174 KB PDF)
Four-position Sample Holder
The muli-position Peltier, featuring a four-position turret and magnetic stirring can accommodate standard cuvettes or microcuvettes. The automated sample holder has a controllable temperature range of -20°C to 105°C.
Powdered Sample Holder
The powdered sample holder head easily adapts to the solid sample base with one thumbscrew adjustment. The sample head can be disassembled for easy sample loading and cleaning.
Download Flyer (146 KB PDF)
Front Face Solid Sample Holder
The front face solid sample holder, capable of both linear and rotational travel, was designed for the measurements of solid compounds, microscope slides, or films. The solid sample holder head mounts onto a base that can be removed easily to substitute a powder sample holder head.
Download Flyer (146 KB PDF)
Titrations are performed to measure a number of biochemical and physical parameters, including binding constants, stoichiometry and kinetics. PTI offers fully automated titration solutions that are integrated into the software. Parameters such as mixing, volume, speed, and calibration are dictated in the software and can be adapted to your needs.
Redesigned for enhanced measurement of quantum yields of solids, films, and powders. We use a 6 inch diameter sphere and attach it directly to the sample chamber on the port opposite the excitation channel. This design minimizes the effect of the excitation, emission, and sampling ports on the accuracy of the measurement.
Download Flyer (432 KB PDF)
Single Cuvette Peltier
The Peltier provides unmatched temperature stability and accuracy over the controllable temperature range of -20°C to 105°C.
The muscle strip is inserted into a standard 1 cm cuvette, and can be used with any cuvette-based fluorescence system having a standard single cuvette holder complete with tension transducer and transducer mounting bracket with micrometer position adjustment.
Cold Finger Dewar
The cold finger dewar is designed to be used with liquid nitrogen as coolant (77 K). It can also be used with organic solvent slushes at discrete temperatures above 77 K.
Download Flyer (126 KB PDF)
Remote Sensing Accessory
The remote sensing accessory allows in vitro or in vivo measurement by means of a quartz bifurcated fiber bundle or Liquid Light Guide.
Selections of Long-pass Filters
Long-pass filter sets provide spectral discrimination for the emission channel and prevent the excitation light from being detected by the PMT. Filters should be selected so they cut off unwanted wavelengths, such the second order. The filters should be used with a filter holder (2 filter holders included with instrument).
The longpass filter set includes 1 inch round filters with cut-off wavelengths of 345, 400, 445, and 515 nm
10 nm bandpass interference filters are available individually or as a set. The peak transmittance wavelengths are 280, 289, 297, 308, 334 nm and from 340 nm to 650 nm at 10 nm increments. The filters should be used with a filter holder (2 filter holders included with instrument). The bandpass filters are especially recommended for:
Neutral Density Filters
Neutral density filter set. The neutral density filter set consists of six 1inch round filters with approximate optical densities of 0.1, 0.3, 0.5, 1.0, 2.0 and 3.0 suitable for a spectral range of 300–2000 nm. The filters should be used with a filter holder (2 filter holders included with instrument). The neutral density filters are used to decrease the signal intensity without changing the width of the emission slit.
The semi-microcuvette cuvette and insert lets the end user save money and time by using smaller volumes of samples while maintaining the correct optical height.
Whether you’re working in biology, medicine, physical or material science, measuring proteins, DNA, FRET and others, the PicoMaster TCSPC lifetime spectrofluorometer is the instrument for you! And with PTI's accessories and system enhancements the PicoMaster the perfect system for any fluorescence lifetime application.
Below are some examples:
- Intrinsic protein fluorescence
- Quantum dots
- DNA binding
- Fluorescence lifetimes
- Protein-protein interactions
- Protein-drug interactions
- Anisotropy decays
- Molecular photophysics
- Semiconductors, glasses and ceramics
|The high dynamic range of PicoMaster greatly facilitates the determination of minor lifetime components in the complex decay of native chlorophyll in photosynthetic system of green leaves|
|PicoMaster is an excellent choice for unraveling structural and dynamic properties of proteins by measuring rotational correlation times from anisotropy decays|
PT. HORIBA Indonesia
Ruko Jalur Sutera Jl. Jalur Sutera Blok 20 A No. 16-17 Tangerang, Banten 15144, Indonesia
Tel: +62 (21) 30448525
Fax: +62 (21) 30448521